PIPages
Labs

Steven Strauss

Oregon State University, OR·Plant Science & Agriculture

Modern biotechnology lets scientists insert and precisely edit genes in crops to improve traits such as stress resistance and product quality. In most woody plants, though, very few cells take up a new gene and grow back into whole modified plants.

Fields

Plant Science & Agriculture

Genetics

Microbiology & Immunology

Topics

Agrobacterium

plant transformation

genetic transformation

Grants
1 known grant, through Aug 2027

What they work on

  1. Screen about 100 sequenced wild Agrobacterium strains for their ability to transform poplar and induce transgenic shoots, and identify the strains with the highest rates.
  2. Analyze why transformation efficiency differs among Agrobacterium strains and poplar genotypes, by studying how high-performing strains interact with specific plant genotypes.
  3. Determine the roles of specific genes on the T-DNA, the DNA segment Agrobacterium transfers into plant cells, in boosting transformation and regeneration in Populus.
  4. Engineer stronger Agrobacterium strains by adding beneficial T-DNA genes to domesticated strains and by domesticating high-performing wild strains.

More about the research

This step, called transformation, is a major bottleneck. Agrobacterium, a soil bacterium that naturally moves a piece of its DNA (the T-DNA) into plant cells, is the standard tool for transformation. This project asks whether natural variation among wild Agrobacterium strains can make transformation of woody plants much more efficient.

Why it matters

Low transformation and regeneration rates block gene-based improvement of most woody crops. More effective Agrobacterium strains could make it practical to engineer trees and other hard-to-modify crops for traits such as stress resistance and quality. This could help breeders and agriculture cope with climate change, and aid functional genomics research in many crop species.

Known grants

From NIH and NSF research grants. Labs often have other funding we can't see.

PlantTransform: Leveraging diversity of wild Agrobacterium to enhance woody plant transformation and regeneration

NSF · Sep 2024 – Aug 2027

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Benjamin Blackman

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Characterize the cell types and gene expression patterns that arise as sunflower tissue attempts to regenerate, using tissue-level analyses and single-nucleus transcriptomics, which reads gene activity in individual cell nuclei.

The school

Oregon State University: 29 labs on PI Pages, $372M a year on research, 238 research doctorates a year.

More about Oregon State University

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